NASC

NASC ID: N848992

Name: WiscDsLox1C10

ABRC stock number: CS848992

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox1C10.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G05230.2 (chr 1 pos 1518188 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG795839.

Wiscseq_DsLox1C10.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G66540.2-UTR3 and AT1G66550.2-P355 (chr 1 pos 24827258 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG795838.

Wiscseq_DsLox1C10.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G50376 and AT3G50380.1-UTR3 (chr 3 pos 18686120 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG795840.

Wiscseq_DsLox1C10.4 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G57660.1 and AT3G57670.1-P355 (at chr 3 pos 21365083 (C/21365083-21365160) on the TAIR10). The sequences' 1-78 bps mapped on the genome, 83-100 bps onto the T-DNA. Pools P 0, R 0, C 2 N 0. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO453282.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox1C10

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
		PFC1	AT1G05230 AT1G66540 AT1G66550 AT3G50376 AT3G50380 AT3G57660 AT3G57670 At1g01860

EMBL accession:

CW792691